anti tropomyosin receptor kinase b trkb (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc anti tropomyosin receptor kinase b trkb
Anti Tropomyosin Receptor Kinase B Trkb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+trka/Phospho-TrkA+(Tyr674%2F675)%2FTrkB+(Tyr706%2F707)+Rabbit+mAb/pm42884630-72-47-53
Average 95 stars, based on 118 article reviews

anti tropomyosin receptor kinase b trkb - by Bioz Stars, 2026-10

95/100 stars

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Western Blot:

Article Title: Nerve growth factor receptor limits inflammation to promote remodeling and repair of osteoarthritic joints
Article Snippet: NGFR-RFP was a gift from Moses Chao (Addgene plasmid # 24092). .. Western blot analysis was performed , using the following antibodies: anti-NGFR, Cell Signaling, catalog # 8238S; anti-phospho-NF-κB p65 (Ser536), Cell Signaling, catalog #3033; anti-NF-κB p65 (D14E12), Cell Signaling, catalog #8242; anti-phospho-IκBα (Ser32), Cell Signaling, catalog # 2859; anti-IκBα, Novus Biologicals, catalog # NB100-56507; anti-p-SMAD1, Cell Signaling, catalog # 9516S; anti-SMAD1, abcam, catalog # ab63356; anti- Phospho-IKKα (Ser176)/IKKβ (Ser177), Cell Signaling, catalog #2078; anti-IKKβ, Cell Signaling, catalog #2678; anti-β-actin, Sigma-Aldrich, catalog # A5441; anti-RANKL, Novus Biologicals, Clone 12A668; anti-TrkA, Cell Signaling, catalog #2505; anti-Trk (pan) (A7H6R), Cell Signaling, catalog #92991. ..

SDS Page:

Article Title: Human TrkA R649W mutation impairs nociception, sweating and cognitive abilities: a mouse model of HSAN IV
Article Snippet: .. Equal amounts of cell extracts were resolved by SDS-PAGE (10%), transferred on nitrocellulose membranes and probed overnight with primary antibodies: anti-TRKA (1:1000, Cell Signaling #2505), anti-phospho-TRKA (1:1000, Cell Signaling #9141), anti-Tubulin (1:10 000, Sigma #T6074). ..

Expressing:

Article Title: Ouabain increases neuronal differentiation of hippocampal neural precursor cells Running title: Role of Ouabain in neurogenesis
Article Snippet: The cells were stained with anti-DCX (1:250; Abcam, Cambridge, MA, USA), AntiGFAP (1:1000; Cell Signaling, USA), anti-O1 (1:250, Thermo, USA), anti β3-tubulin or Tuj1 (1:500, Thermo, USA), and anti-NeuN (1:250; Cell Signaling, USA) to evaluate cell-differentiation. .. To verify the expression of neurotrophin receptors, anti-TrkA (1:250; Cell Signaling Technology, Danvers, MA, USA), anti-TrkB (1:250; Abcam, Cambridge, USA), anti-TrkC (1:250; Cell Signaling Technology), and anti-p75 NTR (1:250; Cell Signaling Technology) were used. .. Anti-beta-catenin was used to verify the expression of nuclear β-catenin (1:200, Cell Signaling, USA), pCREB Ser133 (1:200, Cell Signaling, USA) to the nuclear CREB expression, and finally anti-p65 (1:250, Santa Cruz Biotech, USA) to measure p65 nuclear translocation.After incubation with the primary antibody, the cells were washed three times for 10 min with PBS and incubated for 2 h at room temperature with Alexa Fluor donkey anti-mouse secondary antibody (DAM) 594 and Alexa Fluor Goat anti-rabbit (GAR) 488 (Molecular Probes, Invitrogen, Eugene, OR, USA).

Article Title: Ouabain increases neuronal differentiation of hippocampal neural precursor cells
Article Snippet: The cells were stained with anti-DCX (1:250; Abcam, Cambridge, MA, USA), Anti-GFAP (1:1000; Cell Signaling, USA), anti-O1 (1:250, Thermo, USA), anti βIII-tubulin or Tuj1 (1:500, Thermo, USA), and anti-NeuN (1:250; Cell Signaling, USA) to evaluate cell-differentiation. βIII-tubulin is considered a marker for early mitotic neurons and NeuN a marker of postmitotic neurons ( ). .. To verify the expression of neurotrophin receptors, anti-TrkA (1:250; Cell Signaling Technology, Danvers, MA, USA), anti-TrkB (1:250; Abcam, Cambridge, USA), anti-TrkC (1:250; Cell Signaling Technology), and anti-p75 NTR (1:250; Cell Signaling Technology) were used. .. Anti-beta-catenin was used to verify the expression of nuclear β-catenin (1:200, Cell Signaling, USA), pCREB Ser133 (1:200, Cell Signaling, USA) to the nuclear CREB expression, and finally anti-p65 (1:250, Santa Cruz Biotech, USA) to measure p65 nuclear translocation.

Incubation:

Article Title: Loss of microglia reduces NGF signaling and retinal ganglion cell survival
Article Snippet: .. Membranes were then incubated overnight at 4 °C with primary antibodies diluted in the same blocking buffer at the following concentrations: anti-phospho-TrkA (1:1000; Cell Signaling Technology, Cat. No. 4619), anti-TrkA (1:1000; Cell Signaling Technology, Cat. No. 2510), anti-p75NTR (1:2000; Abcam, Cat. No. Ab52987), anti-RBPMS (1:1000; Abcam, Cat. No. Ab152101), anti-GFAP (1:1000; Abcam, Cat. No. Ab7260), anti-Iba1 (1:5000; Wako Chemicals), and BRN3A (1:500; Santa Cruz Biotechnology, Cat. No. Sc-8429). .. Following primary antibody incubation, membranes were washed and incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (anti-mouse or anti-rabbit; 1:5000; Santa Cruz Biotechnology, Milan, Italy) for 1 hour at room temperature.

Article Title: Depletion of p75NTR in Schwann Cells Driven by Inflammation Mediates Cutaneous Pain in Psoriasis.
Article Snippet: Proteins were separated by SDS-PAGE, transferred to PVDF membranes (Millipore, #ISEQ00010), and blocked with 5% BSA in PBST (0.05% Tween20) for 1 h at room temperature. .. Membranes were incubated overnight at 4◦C with primary antibodies (Anti-S100B (1:1000, sc-393919, Santa Cruz), anti-SOX10(1:1000, sc-365692, Santa Cruz), anti-MBP (1:1000, sc-271524, Santa Cruz), anti- β3-Tubulin (1:1000, #5566, Cell Signaling Technology), anti-p75NTR(1:1000, ab52987, ABcam), anti-NGF(1:1000, A14216, Abclonal), antiGM130 (1:1000, #70767, Cell Signaling Technology), anti-IL-17A (1:1000, 13082-1-AP, Proteintech), anti-p-TrkA (1;1000, #9141, Cell Signaling Technology), anti-TrkA (1:1000, #2505, Cell Signaling Technology), anti-c-Fos (1:1000, #2250, Cell Signaling Technology), anti-p-ERK1/2 (1:1000, #4370, Cell Signaling Technology), anti-ERK1/2 (1:1000, #9102, Cell Signaling Technology), anti-p-AKT (1:2000, #4060, Cell Signaling Technology), antiAKT (1:1000, #9272, Cell Signaling Technology), anti-GAPDH (1:5000, 60004-1-Ig, Proteintech), anti-KEAP1(1:1000, 10503-2-AP, Proteintech), anti-Nrf2 (1:1000, #20733, Cell Signaling Technol- 15 of 18 e C om m ons L icense o a i a g b ( 4 T o a t G G S t w ( i e w r b w f a 4 C e E D m m ( C 4 A w d c u w i w e A Y Z 1 nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202523189 by IN A SP - N E PA L , W iley O nline L ibrary on [06/04/2026]. .. Dermal tissue was rinsed in DMEM, minced, and digested in 2 mg/mL collagenase in DMEM (5 mL) at 37◦C for 2.5 h with Advanced Science, 2026 e g r d c c w a i 1 c s β w c o 4 P c c t S t r N e a p c 4 C t m 1 g C a s β ( A w p o ( A c t ( w t A s C i p A nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202523189 by IN A SP - N E PA L , on [06/04/2026]

Blocking Assay:

Cell-Signaling:

Article Title: ProNGF promotes brain metastasis through TrkA/EphA2 induced Src activation in triple negative breast cancer cells
Article Snippet: .. The following primary antibodies were used: anti-beta-Actin (Sigma-Aldrich, A2066, rabbit), anti-pTrkA (Tyr674/675, Cell Signaling, 9141, rabbit), anti-TrkA (Cell Signaling, 2510, rabbit), anti-Src (Cell Signaling, 2123, rabbit), anti-pSrc (Tyr416, Cell Signaling, 2021, rabbit). .. After washing (TBST 0.1% (v/v), 5 × 5 min, 20 °C), the membranes were incubated with the secondary antibody (HRP-linked Anti-rabbit, Goat, 1/5000, 7074, Cell signaling) diluted in TBST (0.1% (v/v)).

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